منابع مشابه
Cell surface display of a single-chain antibody for attaching polypeptides.
To provide an efficient means of coupling proteins, peptides and other suitable moieties to cells, we have constructed a retroviral expression vector for cell surface display of a single-chain antibody (scFv) against the hapten 4-ethoxymethylene-2-phenyl-oxazo-line-5-one (phOx). The hapten phOx can be easily conjugated to primary amino and sulfhydryl groups, thus providing points of attachment ...
متن کاملEscherichia coli surface display of single-chain antibody VRC01 against HIV-1 infection.
Human immunodeficiency virus type 1 (HIV-1) transmission and infection occur mainly via the mucosal surfaces. The commensal bacteria residing in these surfaces can potentially be employed as a vehicle for delivering inhibitors to prevent HIV-1 infection. In this study, we have employed a bacteria-based strategy to display a broadly neutralizing antibody VRC01, which could potentially be used to...
متن کاملSelection of single-chain variable fragments specific for Mycobacterium tuberculosis ESAT-6 antigen using ribosome display
Objective(s): Tuberculosis (TB) is still one of the problematic infectious diseases in developing countries, especially in Iran. In the present study, we applied ribosome display technique to select single chain variable fragments (scFvs) specific for the 6-kDa early secretory antigenic target (ESAT-6) antigen of Mycobacterium tuberculosis from a mouse scFv library. Materials and Methods: The g...
متن کاملBacterial phage receptors, versatile tools for display of polypeptides on the cell surface.
Four outer membrane proteins of Escherichia coli were examined for their capabilities and limitations in displaying heterologous peptide inserts on the bacterial cell surface. The T7 tag or multiple copies of the myc epitope were inserted into loops 4 and 5 of the ferrichrome and phage T5 receptor FhuA. Fluorescence-activated cell sorting analysis showed that peptides of up to 250 amino acids w...
متن کاملoptimization of a single-chain antibody fragment overexpression in escherichia coli using response surface methodology
human epidermal growth factor receptor (her) family plays an important role in various types of cancers. as a result, antibodies against her and the mechanism of antigen-antibody binding action are under active investigation. we previously constructed a single-chain variable fragment (scfv) against her2, i.e. anti-her2 scfv, for expressing in the escherichia coli . in the present study, we repo...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BioTechniques
سال: 1996
ISSN: 0736-6205,1940-9818
DOI: 10.2144/96214st02